FIG. 3.

Hog1-dependent gene expression in response to sodium arsenite. (A) Wild-type and hog1Δ strains were treated with 1 mM sodium arsenite for 1 h. After total RNA extraction, Arr1, Arr3, Ycf1, and Gpd1 mRNAs were quantified using quantitative reverse transcription-PCR (RT-PCRQ). Bars show the ratio of treated/untreated for each strain. Two graphs are shown for better observation of scale differences between experiments. Standard deviation of three experiments is shown. (B) Transcription factors and sensitivity to arsenite. Serial dilutions of the wild type and hog1Δ, arr1Δ, sko1Δ, msn2Δmsn4Δ, hot1Δ, and smp1Δ mutants were plated in rich media and media containing sodium arsenite. Pictures were taken after 2 to 3 days at 24°C. The double mutant msn2Δmsn4Δ was kindly provided by Raul García. wt, wild type.