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HPLC elution profiles of the C. tepidum wild type (WT) and mutants unable to synthesize various modified carotenoids. Peak 1, OH-chlorobactene (550.4 Da); peak 2, OH-chlorobactene glucoside (729.4 Da); peak 3, OH-chlorobactene glucoside laurate (894.6 Da); peak 4, chlorobactene (532.4 Da); peak 5, γ-carotene (536.4 Da); peak 6, 1′,2′-dihydrochlorobactene (534.5 Da). Elution of carotenoid species was monitored at 491 nm.
