TABLE 1.
Sequences of primers used for construction of mutants and confirmation of segregation
| Primer name | Primer sequencea | Purpose |
|---|---|---|
| CT1987 F1 | CAG GAA GCT TAT GAA AAG TGC | Confirming segregation |
| CT1987 R1 | CGC GAG CTA CCG GAG GGT TGG | Confirming segregation |
| CT1987 F2 | TGG AGT CGA CGA AAG CAT CC | Amplifying the right flank of inactivation construct |
| CT1987 R2 | CTG CCT TAG GTA CCG CGG TTT CG | Amplifying the left flank of inactivation construct |
| CT1987 F3 | CCA GAT TTT TCA CCA CAG GCT CC | Amplifying the left flank of inactivation construct |
| CT1987 R3 | TTC CAC TTT TCC GTC CAG AGC C | Amplifying the right flank of inactivation construct |
| CT0967 F2 | GAA TGC ACT GTC GAC TTT TCC G | Amplifying the right flank of inactivation construct |
| CT0967 R2 | AGT GAA GAA CTG GTA CCT GAA GAG | Amplifying the left flank of inactivation construct |
| CT0967 F3 | TCA TCA ATG CGT GCG ACA ACT | Amplifying the left flank of inactivation construct |
| CT0967 R3 | CAT CGT TTC TTC TAC GGA TTT ACC C | Amplifying the right flank of inactivation construct |
a
KpnI sites are underlined, and SalI sites are underlined and in italics.