TABLE 2.
Glycosyl compositions of ethanol-precipitated bacterial culture supernatants and of EPS (HMW fraction) purified by gel filtration
| Strain | Glycosyl composition (mol%)a
|
|||
|---|---|---|---|---|
| Gal | Glc | GlcA | PvGal | |
| Culture supernatant precipitated with ethanolb | ||||
| NGR234 | 20.2 | 52.3 | 11.3 | 8.9 |
| NGRΩexoF | 46.6 | 53.4 | 0c | 0c |
| NGRΩexoK | 21.2 | 46.4 | 6.0 | 12.3 |
| NGRΩexoL | 54.8 | 45.2 | 0 | 0 |
| NGRΩexoP | 38.8 | 61.2 | 0 | 0 |
| NGRΩexoQ | 38.1 | 61.9 | 0 | 0 |
| NGRΩexoY | 63.6 | 36.4 | 0 | 0 |
| NGRΩexoZ | 19.9 | 49.9 | 9.7 | 5.4 |
| Purified EPS (HMW fraction) after Sephadex G-75 chromatography | ||||
| NGR234 | 16.4 | 51.4 | 13.5 | 12.2 |
| NGRΩexoK | 21.9 | 53.4 | 9.4 | 9.9 |
| NGRΩexoZ | 14.9 | 51.2 | 13.6 | 8.8 |
a
Gal, galactose; Glc, glucose; GlcA, glucuronic acid; PvGal, 4,6-pyruvylated galactose.
b
Ethanol-precipitated culture supernatants contained variable amounts of xylose and mannose. All samples also contained trace amounts of 3-deoxy-d-manno-2-octulosonic acid and galacturonic acid.
c
≤0.01 mol% based on a detection limit of ∼1 ng by GC-MS.