TABLE 2.
Quantitative RT-PCR analysis of mRNA levels in tlc1 through tlc5
| Gene | Reaction efficiencya
|
 b
|
Avg ΔCT from that of tlc1c | Expression relative to that of tlc1d | ||
|---|---|---|---|---|---|---|
| Sample 1 | Sample 2 | Sample 1 | Sample 2 | |||
| tlc1 | 3.1 | 3.6 | 25.5 | 25.7 | 1.0 | |
| tlc2 | 2.9 | 3.3 | 27.1 | 27.9 | −1.9 | 0.3 |
| tlc3 | 3.5 | 4.0 | 27.5 | 27.3 | −1.8 | 0.3 |
| tlc4 | 3.4 | 3.9 | 23.5 | 22.2 | 2.8 | 6.7 |
| tlc5 | 3.1 | 3.9 | 25.8 | 25.3 | 0.1 | 1.1 |
a
As described in Materials and Methods, quantitative PCR was performed in duplicate on serial dilutions of the tlc-specific cDNA, and the CT was plotted against the log template concentration from two biological replicates. Linear regression analysis determined the reaction efficiency, where a theoretical slope of 3.3 is expected.
b
, the ordinate intercept of the linear regression.
c
The 
values from the two samples were averaged and used to calculate the difference in CT (ΔCT) from that of tlc1.
d
The ΔCT values were used to determine expression relative to that of tlc1 where change (n = fold) was 
.