Distribution of DnaN-EGFP foci in mycelium of S. coelicolor M145 and the ΔD78 mutant. (A) S. coelicolor strains M145 and ΔD78 carrying dnaN-egfp were grown for 26 and 42 h. The images show overlays of two fluorescence signals: DnaN-EGFP foci and cell wall stained with wheat germ agglutinin-tetramethylrhodamine conjugate. Bars, 5 μm. The arrows indicate types of fluorescence (green arrows, compact foci; lilac arrows, diffuse foci; yellow arrows, dispersed foci), and the red arrowheads indicate boundaries of compartments. (B) Fractions of compartments of S. coelicolor strains M145 and ΔD78 harboring compact foci (580 hyphal compartments of each strain analyzed were examined). WT, wild type.