TABLE 3.
Cosmids and plasmids used in this study
| Plasmid or cosmid | Relevant characteristics | Source or reference |
|---|---|---|
| pGEM-T Easy | bla, T vector for cloning PCR-amplified fragments | Promega |
| pGEM-T Easy D78 | pGEM-T Easy derivative containing cluster of D78 | This study |
| pGEM-T Easy H24 | pGEM-T Easy derivative containing cluster of H24 | This study |
| pGEM-T Easy H69 | pGEM-T Easy derivative containing cluster of H69 | This study |
| pWHM3 | tsr bla lacZα, bifunctional vector derivative of pIJ486 | 51 |
| pWHM3 D78 | pWHM3 derivative containing additional cluster of D78 | This study |
| pWHM3 H24 | pWHM3 derivative containing additional cluster of H24 | This study |
| pWHM3 H69 | pWHM3 derivative containing additional cluster of H69 | This study |
| pGEXDnaA(BD) | Expression vector, BD of S. coelicolor DnaA protein fused to glutathione S-transferase | 34 |
| pLEXDnaAx6His | Expression vector, isolation of S. coelicolor DnaA protein | 33 |
| H24ΔDnaAbox::apra | Cosmid H24 ΔDnaAboxes32620<>32955::aac(3)IV | This study |
| D78ΔDnaAbox::apra | Cosmid D78 ΔDnaAboxes9635<>9885::aac(3)IV | This study |
| H24ΔDnaAbox::Swa_relig | Cosmid H24 ΔDnaAboxes32620<>32955::SwaI religation, kan::vph | This study |
| D78ΔDnaAbox::Swa_relig | Cosmid D78 ΔDnaAboxes9635<>9885::SwaI religation, kan::vph | This study |