FIG. 1.

Diagram of i31 library and conjugation phenotypes of traI::i31 mutants. The bar shows the length of the TraI protein, with four regions highlighted by different patterns: gray, relaxase domain; lined, region of unknown function; white, helicase domain; spotted, putative protein-protein interaction domain. Each allele (except the Q794 allele) was introduced on a pTrc-based vector to a CAW400 pOXΔtraI donor, and the resulting strains were assayed for their abilities to transfer pOXΔtraI to an F⁻ recipient. For the Q794 allele, the complementation phenotype was assayed in an XK1502 F′ΔI donor. Mutants are listed by position of i31 insert, and their phenotypes relative to the ΔtraI/p99I⁺ control donor are shown by the labeled vertical lines. The pOXΔtraI/p99I⁺ control donor had a conjugation frequency of 8.0 × 10⁻³ transconjugants/donor.