FIG. 3.

Complementation and dominance phenotypes of traI and traI::i31 alleles in conjugation. XK1502 donors carrying different combinations of F′₄₂- and pTrc-derived plasmids (as indicated) were assayed for their ability to transfer an F′lac plasmid into an F⁻ recipient. Each bar represents the conjugative efficiency of one donor strain. Similarly, colored bars represent the four types of experiments: gray, complementation of F′ΔI in trans; black, phenotype of traI::i31 mutants in cis; white, dominance of p99I⁺ or p99traI::i31s over the F′₄₂; hatched, dominance of p99I⁺ over F′traI::i31s. Alleles are listed by position of insert (and functional class); “wild type” denotes wild-type traI. An F′₄₂/pTrc99A donor had the same conjugation frequency as did a donor carrying the F′₄₂ alone. Error bars represent ±standard errors of the means of results of two to four independent biological replicates.