TABLE 2.
Conjugative dominance of TraI-LacZ fusionsa
| Position of fusion | Relaxase domain | Restrictive domain | Helicase domain | Interaction domain | Fraction of positive control transfer frequencyb |
|---|---|---|---|---|---|
| Q6 | − | − | − | − | 0.77 |
| Q369 | + | − | − | − | 0.99 |
| L930 | + | + | − | − | 0.79 |
| V996 | + | + | − | − | 0.78 |
| V1130 | + | + | − | − | 0.84 |
| L1208 | + | + | − | − | 0.81 |
| E1685 | + | + | + | − | 0.59 |
| L1753 | + | + | + | ? | 0.18 |
a
+, domain is present; −, domain is absent or disrupted; ?, domain may be disrupted.
b
Positive control strain was XK1502 F′42 pTrc99A. Values shown are means of two to four independent biological replicates.