TABLE 1.
Plasmids and strains used in this study
| Plasmid or strain | Description or genotypea | Source or referenceb |
|---|---|---|
| Plasmids | ||
| pRB381 | E. coli-Bacillus shuttle vector for translational fusion with β-galactosidase gene; ′lacZ Kmr Apr | BGSC (4) |
| pPZ | pRB381 bearing 850-bp insert (−817 to +33) containing ScoC and SinR binding sites, epr promoter, RBS, and ATG in translation fusion with lacZ gene | Laboratory stock |
| pSZ | pRB381 bearing 455-bp insert (−422 to +33) containing ScoC and SinR binding sites, epr promoter, RBS, and ATG in translation fusion with lacZ gene | This study |
| pHZ | pRB381 bearing 341-bp insert (−312 to +33) containing epr promoter, RBS, and ATG in translation fusion with lacZ gene | This study |
| pSHZ | pSZ with mutated ScoC binding site | This study |
| pSRZ | pSZ with mutated SinR binding site | This study |
| pS200Z | pSZ with 200-bp DNA insertion between ScoC and SinR binding sites | This study |
| Strains | ||
| 168 | trpC2 | BGSC |
| 168-SZ | B. subtilis 168 bearing pSZ | This study |
| 168-HZ | B. subtilis 168 bearing pHZ | This study |
| 168ΔH | trpC2 scoC::Spr | Laboratory stock |
| 168ΔR | trpC2 sinR::Cmr | Laboratory stock |
| 168ΔH-SZ | B. subtilis 168ΔH bearing pSZ | This study |
| 168ΔR-SZ | B. subtilis 168ΔR bearing pSZ | This study |
| 168-SHZ | B. subtilis 168 bearing pSHZ | This study |
| 168-SRZ | B. subtilis 168 bearing pSRZ | This study |
| 168-S200Z | B. subtilis 168 bearing pS200Z | This study |
a
Kmr, kanamycin resistance; Apr, ampicillin resistance; Spr, spectinomycin resistance; Cmr, chloramphenicol resistance.
b
BGSC, Bacillus Genetic Stock Center.