TABLE 2.
Primers used in this study
| Primer | Sequence (5′ to 3′)a | Restriction site(s)b |
|---|---|---|
| KKR28 | GGTAAAGCTTAGGATATCCGAGC | HindIII then EcoRV |
| KKR36 | CTTAGGATCCATGATTCATCTCC | BamHI |
| KKR67 | CGTAAGCTTAATCAGAAGACGCGC | HindIII |
| KKR103 | CGACTGCAGCGGCATGGTCAGGAT | PstI |
| KKR127 | CGCTTTGCGTGACGGATTATC | |
| KKR215 | GCACCCGGGCAGCGGCATGGTCA | SmaI |
| KKR216 | GCACCCGGGATCGGGTCTGTCTTC | SmaI |
| KKR217 | ATTTATAACTGCCCATGAAATCAGC | SspI |
| KKR218 | ATTAAAAGTGGGCCGATAAGGTC | SspI |
| KKR253 | GCAGCTAGCAGCTCGTTAAAATCAAG | NheI |
| KKR254 | CGAGCTAGCTTCCGGGGTATGCTG | NheI |
a
Nucleotides in boldface are complementary to the genome sequence.
b
Underlined in the corresponding sequence.