TABLE 2.
Insertion site identification for M. pneumoniae SGA mutants
| Mutanta | Siteb | Descriptionc | Homology
|
||
|---|---|---|---|---|---|
| M. genitalium | M. gallisepticum | M. mobile | |||
| Transport and metabolism | |||||
| MPN114-566 | 150449 | Cat, carnitine O-acetyltransferase, hypothetical | Noned | None | None |
| MPN308-274 | 363224 | Amino acid transporter | MG225 | MGA0287 | MMOB2220 |
| MPN320-30 | 380633 | ThyA, thymidylate synthase | MG227 | MGA0699 | MMOB0340 |
| MPN333-32 | 393007 | Predicted ABC transport system, permease component | None | MGA1302 | None |
| MPN333-614 | 394752 | Predicted ABC transport system, permease component | None | MGA1302 | None |
| MPN335-38 | 396248 | Predicted ABC transport system, permease component | None | MGA1302 | None |
| MPN335-377 | 397263 | Predicted ABC transport system, permease component | None | MGA1302 | None |
| MPN335-595 | 397917 | Predicted ABC transport system, permease component | None | MGA1302 | None |
| MPN407-137 | 489736 | Predicted lipase/acylhydrolase | None | None | None |
| MPN493-20 | 600912 | UlaD, 3-keto-L-gulonate-6-phosphate decarboxylase | None | None | None |
| MPN509-145 | 621408 | Mycoplasma-specific export protein | MG288 | None | None |
| MPN510-42 | 624124 | Mycoplasma-specific export protein | MG288 | None | None |
| DNA/protein modification | |||||
| MPN107-68 | 139495 | Predicted DNA methylase | None | None | None |
| MPN107-152 | 139746 | Predicted DNA methylase | None | None | None |
| MPN110-112 | 143608 | Predicted restriction endonuclease | None | None | None |
| MPN153-35 | 202490 | Superfamily I DNA/RNA helicase | MG140 | MGA0793 | None |
| MPN247-100 | 298631 | PrpC, serine/threonine protein phosphatase | MG108 | MGA0461 | MMOB5580 |
| MPN294-96 | 350687 | Similar to ATP-independent intracellular protease | None | MGA0998 | None |
| MPN342-324 | 408833 | Restriction-modification system methyltransferase | None | MGA0537 | None |
| MPN342-362 | 408949 | Restriction-modification system methyltransferase | None | MGA0537 | None |
| MPN607-64 | 727792 | MsrA, peptide methionine sulfoxide reductase | MG408 | MGA0571 | MMOB4950 |
| Unknown function | |||||
| MPN083-62 | 104127 | Uncharacterized mycoplasma-specific lipoprotein | MG067 | MGA1162 | None |
| MPN104-29 | 134672 | Uncharacterized mycoplasma-specific protein | None | None | None |
| MPN254-70 | 305278 | CinA, uncharacterized protein (competence induced) | MG115 | None | None |
| MPN281-61 | 333465 | Uncharacterized mycoplasma-specific lipoprotein | MG185 | MGA0674 | None |
| MPN311-22 | 371006 | P41, uncharacterized mycoplasma-specific protein | MG218.1 | None | None |
| MPN311-161 | 371454 | P41, uncharacterized mycoplasma-specific protein | MG218.1 | None | None |
| MPN358-485 | 429136 | Uncharacterized mycoplasma-specific protein | MG255 | None | None |
| MPN359-12 | 429550 | Hypothetical mycoplasma-specific protein | MG256 | None | None |
| MPN359-54 | 429675 | Hypothetical mycoplasma-specific protein | MG256 | None | None |
| MPN359-93 | 429792 | Hypothetical mycoplasma-specific protein | MG256 | None | None |
| MPN359-130 | 429904 | Hypothetical mycoplasma-specific protein | MG256 | None | None |
| MPN359-131 | 429906 | Hypothetical mycoplasma-specific protein | MG256 | None | None |
| MPN359-132 | 429909 | Hypothetical mycoplasma-specific protein | MG256 | None | None |
| MPN359-160 | 429994 | Hypothetical mycoplasma-specific protein | MG256 | None | None |
| MPN359-175 | 430040 | Hypothetical mycoplasma-specific protein | MG256 | None | None |
| MPN376-744 | 450763 | Uncharacterized mycoplasma-specific protein | None | None | None |
| MPN376-626 | 451116 | Uncharacterized mycoplasma-specific protein | None | None | None |
| MPN376-335 | 451988 | Uncharacterized mycoplasma-specific protein | None | None | None |
| MPN387-2 | 465051 | Uncharacterized mycoplasma-specific protein | MG269 | None | None |
| MPN403-114 | 485469 | Hypothetical mycoplasma-specific protein | MG284 | MGA1027e | MMOB2170 |
| MPN404-42 | 485600 | Hypothetical mycoplasma-specific protein | MG285 | MGA1029e | None |
| MPN524-74 | 645829 | Uncharacterized mycoplasma-specific protein | None | None | None |
| MPN582-82 | 704868 | Uncharacterized mycoplasma-specific lipoprotein | MG395 | MGA1162 | None |
| MPN614-33 | 738065 | Hypothetical mycoplasma-specific protein | MG414 | None | None |
| MPN634-134 | 760807 | Uncharacterized mycoplasma-specific protein | None | None | None |
| MPN648-107 | 773430 | Hypothetical mycoplasma-specific protein | MG441 | None | None |
a
ORF numbering according to reference 8.
b
Nucleotide after which the transposon inserted (this study).
c
Based on reference 8 and updated by current BLAST analysis.
d
Homology assigned when greater than 25% sequence identity exists over at least 50% of the M. pneumoniae protein, except as indicated.
e
Twenty-five percent identity over greater than 40% of the protein sequence.