TABLE 2.
Microarray analysis of stage II and stage III sporulation genesa
| Gene | Expression ratio
|
Transcription mode | |
|---|---|---|---|
| AWS96 | AWS141 | ||
| spoIIAA | 7.5 | 9.7 | Spo0A dependent |
| spoIIAB | 7.7 | 14.4 | Spo0A dependent |
| spoIIB | 5.1 | 4.3 | Spo0A dependent |
| spoIIGA | 2.6 | 9.0 | Spo0A dependent |
| sigG | 4.7 | 5.6 | σF dependent |
| spoIIQ | 3.3 | 5.4 | σF dependent |
| spoIIR | 2.5 | 2.1 | σF dependent |
| spoIIIAC | 2.0 | 3.1 | σE dependent |
| spoIIIAB | 1.9 | 3.7 | σE dependent |
| spoIIIAE | 3.0 | 3.1 | σE dependent |
| spoIIIAH | 1.7 | 2.2 | σE dependent |
| spoIIID | 15.2 | 4.6 | σE dependent |
| spoIVA | 10.0 | 6.9 | σE dependent |
| spoIVFA | 3.4 | 4.2 | σE dependent |
| spoVID | 3.8 | 2.5 | σE dependent |
| spoVR | 3.1 | 4.2 | σE dependent |
a
RNA was isolated at h 4 of stationary phase from wild-type [AWS96 (citB+)] or Aco1-expressing [AWS141 (citB::spc Pspac*-aco1)] cells. RNA was labeled with Cy3 and mixed with RNA isolated during the exponential growth phase from wild-type (AWS96) cells that had been labeled with Cy5. The mixed RNA was annealed to arrays of 4,106 B. subtilis open reading frames. The values reported are the ratios of annealing of T4 RNA to exponential-phase RNA. For details of the microarray analysis, see reference 19.