Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (20%) of B. subtilis cells producing the YtvA protein (stained with Coomassie brilliant blue). Lane 1, molecular mass standards; lane 2, extracts from strain PB198/pYtvA in the absence of IPTG, lane 3, extracts from strain PB198/pYtvA in the presence of IPTG (1 mM final concentration), both harvested 3 h after induction.