FIG. 3.

A prb mutant is affected in the uptake of PB at high osmolarity. Cells of strains Rm1021 (wild type [WT]) and S7 (prb) grown to mid-log phase in MCAA medium were subjected to a 1.5-h NaCl treatment (final concentration of 0.3 M) in the presence or absence of 100 μM PB or diluted in MCAA medium with 0.3 M NaCl supplemented or not supplemented with 100 μM PB and grown for 15 h to mid-log phase. Uptake measurements of PB were done at a final concentration of 40 μM. Results are means of duplicates from three independent experiments, and standard deviations did not exceed 15%.