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PB uptake activity is severely impaired in a prb betS double mutant. Cells were grown for 18 h in MCAA medium containing 0.3 M NaCl or 0.3 M NaCl and 100 μM PB. Assays for the uptake of PB were done at a final concentration of 40 μM using cells from strains Rm1021 (wild type), RmUNA369 (betS), S7 (prb), and RmUNA370 (prb betS). The results are means of duplicates from three independent cultures; standard deviations from the means are also shown.
