TABLE 1.
Bacterial strains and plasmids used in this study
| Strain, plasmid, or transposon | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| S. meliloti | ||
| Rm1021 | SU47 str-21 Smr | 37 |
| Rm5000 | SU47 rif-5 Rifr | 20 |
| Rm1021-S1 (S1) | Rm1021::Tn5 pRL1063a Smr Neor | 46 |
| Rm1021-S2 (S2) | Rm1021::Tn5 pRL1063a Smr Neor | 46 |
| Rm1021-S3 (S3) | Rm1021::Tn5 pRL1063a Smr Neor | 46 |
| Rm1021-S4 (S4) | Rm1021::Tn5 pRL1063a Smr Neor | 46 |
| Rm1021-S5 (S5) | Rm1021::Tn5 pRL1063a Smr Neor | 46 |
| Rm1021-S6 (S6) | Rm1021::Tn5 pRL1063a Smr Neor | 46 |
| Rm1021-S7 (S7) | Rm1021::Tn5 pRL1063a Smr Neor | 46 |
| Rm1021-S8 (S8) | Rm1021::Tn5 pRL1063a Smr Neor | 46 |
| RmUNA369 | Rm1021 betS::Ω Smr Spcr | This work |
| RmUNA370 | S7 betS::Ω Smr Spcr Neor | This work |
| RmHY220 | Rm5000 hutX::Ω | 6 |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 53 |
| MT616 | MT607 pRK600 | 21 |
| Plasmids | ||
| pRK600 | ColE1 replicon with RK2 transfer region; Cmr | 21 |
| pH 45-Ω | pBR322 derivative with interposon Ω; Ampr Smr Spcr | 49 |
| pSup 202 | ColE1 replicon; Mob+ Tcr Ampr Cmr | 56 |
| pSBI39 | Derivative of pSup202 with an S. meliloti 3.4-kb insert containing the betS gene | 7 |
| pSM28 | pSBI39 betS::Ω (KpnI insertion) | This work |
| pSIF7 | 10-kb EcoRI fragment of Rm1021-S7 containing prbB::Tn5 pR1063a; Kmr | This work |
| pSIF5 | 4.3-kb EcoRI fragment of Rm1021-S5 containing prbB::Tn5pR1063a; Kmr | This work |
| pSIF3 | 4.3-kb EcoRI fragment of Rm1021-S3 containing prbC::Tn5 pR1063a; Kmr | This work |
| Transposon | ||
| Tn5pRL1063a | Tn5 derivative; promoterless luxAB; oriV Kmr | 63 |
a
Ampr, ampicillin resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Neor, neomycin resistance; Rifr, rifampicin resistance; Spcr, spectinomycin resistance; Smr, streptomycin resistance; Tcr, tetracycline resistance.