TABLE 1.
Strain | OD600 | Major thiol content (nmol/109 cells)
|
||
---|---|---|---|---|
MSH | Cys-GlcN-Ins | fCys-GlcN-Ins | ||
Wild type | 0.28 | 27 | <0.02 | <0.02 |
Wild type | 0.47 | 27 | <0.02 | <0.02 |
ΔmshD mutant | 0.22 | <0.03 | 0.71 | 1.36 |
ΔmshD mutant | 1.07 | 0.59 | 4.8 | 4.4 |
Wild type + pCV125::mshD | 0.36 | 31 | <0.02 | <0.02 |
Wild type + pCV125::mshD | 0.92 | 35 | <0.02 | <0.02 |
ΔmshD mutant + pCV125::mshD | 0.42 | 39 | <0.02 | <0.02 |
ΔmshD mutant + pCV125::mshD | 1.01 | 46 | <0.02 | <0.02 |