TABLE 2.

Thiol levels in wild-type and ΔmshD strains of M. tuberculosis and M. smegmatis

Thiol	Strain	Thiol level (nmol per 109 cells)
		M. tuberculosisa (mean ± SEM)					M. smegmatisb (range)
		Day 3	Day 7	Day 17	Day 33	Day 60
MSH	Wild type	18 ± 1	22 ± 2	64 ± 5	75 ± 5	65 ± 11	20-28
	ΔmshD	0.25 ± 0.23	0.21 ± 0.13	0.65 ± 0.10	1.7 ± 0.3	1.9 ± 0.3	0.07-0.32
Cys-GlcN-Ins	Wild type	≤0.02	≤0.01	0.038 ± 0.003	0.06 ± 0.03	0.09 ± 0.03	<0.02
	ΔmshD	2.7 ± 0.2	3.4 ± 0.14	11 ± 3	11 ± 3	5.8 ± 0.4	1.0-3.3
fCys-GlcN-Ins	Wild type	<0.1	<0.1	<0.1	<0.1	<0.1	<0.02
	ΔmshD	4.9 ± 0.3	2.7 ± 0.1	8.9 ± 0.8	6.6 ± 0.5	17 ± 2	3.8-7.2
succCys-GlcN-Ins	Wild type	<0.01	<0.01	<0.01	<0.01	<0.01	<0.02
	ΔmshD	<0.02	0.03 ± 0.03	0.14 ± 0.01	0.38 ± 0.06	<0.02	0.06-0.36
Cys	Wild type	≤0.06	≤0.04	≤0.03	≤0.03	≤0.03	0.14-0.46
	ΔmshD	≤0.06	0.17 ± 0.01	0.21 ± 0.02	0.21 ± 0.05	0.12 ± 0.01	0.04-0.16
CoA	Wild type	≤0.8	≤0.8	2.1 ± 0.3	4.1 ± 0.2	5.1 ± 0.6	0.7-5
	ΔmshD	1.3 ± 0.2	≤0.8	2.2 ± 0.1	2.4 ± 1.7	4.9 ± 0.5	1.0-4

^aMeans and standard errors from triplicate determinations.

^bRange of values calculated from data in reference 16, based upon the formula 1 μmol per g residual dry weight = 2 nmol per 10⁹ cells.