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Effect of pH upon thiol content of wild-type and ΔmshD M. tuberculosis strains grown in Middlebrook 7H9 medium
| pH | Wild type
|
ΔmshD
|
||||||
|---|---|---|---|---|---|---|---|---|
| OD600 | nmol per 109 cells
|
OD600 | nmol per 109 cells
|
|||||
| MSH | CoA | MSH | Cys-GlcN-Ins | fCys-GlcN-Ins | CoA | |||
| 7.0 | 0.43 | 14 ± 1 | 0.19 ± 0.03 | 0.41 | 0.25 ± 0.16 | 0.83 ± 0.02 | 1.65 ± 0.04 | 0.15 ± 0.05 |
| 5.5 | 0.63 | 26 ± 3 | 0.26 ± 0.02 | 0.36 | 0.13 ± 0.02 | 1.6 ± 0.2 | 5.5 ± 0.3 | 0.33 ± 0.03 |
