TABLE 2.
Hydrogen production, doubling times, and protein yields of R. palustris wild type and hydrogen uptake mutants grown under nitrogen-fixing conditionsa
| Carbon source utilized (concn) | O/H ratiob | H2 produced (μmol/mg protein)
|
Doubling time (h)
|
Protein yield (μg/10-ml culture)
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| CGA009 (hupV) | CGA010 (wild type) | CGA550 (hupS) | CGA009 (hupV) | CGA010 (wild type) | CGA550 (hupS) | CGA009 (hupV) | CGA010 (wild type) | CGA550 (hupS) | ||
| Malate (10 mM) | 1.25 | 120 ± 20 | <1 | 110 ± 40 | 13.5 ± 1.3 | 11.5 ± 0.7 | 15.0 ± 2.3 | 500 ± 40 | 740 ± 20 | 600 ± 100 |
| Succinate (10 mM) | 1.0 | 140 ± 45 | 25 ± 35 | 125 ± 2 | 11.5 ± 0.2 | 9.5 ± 0.5 | 11.0 ± 0.7 | 740 ± 170 | 1150 ± 80 | 820 ± 200 |
| Acetate (20 mM) | 0.67 | 200 ± 45 | 110 ± 60 | 210 ± 25 | 7.5 ± 0.4 | 6.5 ± 0.8 | 8.0 ± 0.3 | 800 ± 120 | 970 ± 80 | 780 ± 250 |
a
Data were acquired during stationary phase after all available carbon had been utilized. Data are averages from three different experiments, plus or minus standard deviations.
b
The O/H ratio is the ratio of the number of oxygen atoms to the number of H atoms in the carbon source being tested.