Figure 4.

Correlation of a series of reaction rate constants and midpoint potentials of apo-lactate oxidase reconstituted with FMN analogs. The numbering of the data points is the same as in Fig. 1 and Table 1. (a) Reductive half reaction with l-lactate (triangles) or l-mandelate (circles). (b) Oxidative half reaction with molecular oxygen. (c) Sulfite binding reaction (closed triangles); calculated second order rate constant for reaction with l-lactate, from the relationship k_red/K_d′ (open triangles). (d) Sulfite release reaction. The values of k_off were determined as described in ref. 2.