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Accumulation of GerE protein in mutant and wild-type (wt) strains. Extracts of stationary-phase cultures of strains AWS144 (citB+), AWS133 (citB5), and EUDC9901 (gerE::kan) harvested at the indicated time points (Tn = n h after the end of the exponential growth phase) were assayed for GerE protein by immunoblotting after separation by SDS-15% PAGE. GerE (8 kDa; indicated by the arrow) was the fastest-migrating band that reacted with antibody.
