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Immunoblot with antibody to σK. AWS144 (citB+ [wt]), AWS133 (citB5), and JHBS5 (citB+ ΔsigK [ΔK]) were grown in DSM at 37°C. Samples were isolated at the time points indicated (T, indicated number of hours after entry into stationary phase). Crude cellular extracts were subjected to SDS-PAGE and analyzed by immunoblotting with antibody specific to the σK protein.
