Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Gel mobility shift assays of aconitase binding to gerE mRNA. The gerE mRNA 3′ UTR (A), the gerE antisense RNA 3′ UTR (B), and the fliT antisense RNA 3′ UTR (C) were synthesized and radiolabeled by in vitro transcription. Radiolabeled RNA was incubated without or with increasing concentrations of pure B. subtilis His10-aconitase, as indicated, and then analyzed for complex formation by gel mobility shift assay.
