Table 1.

Products for the reaction of the SOR–ferrocyanide complex with a 55 μM concentration of O₂^·̅, generated by γ-ray radiolysis (88 Gy at 17.6 Gy/min) at pH 7.6

Ferrocyanide, molar equivalent	[H2O2]final,* μM	[H2O2]corr,† μM	[SOR] oxidized,‡ μM	[Ferricyanide],‡ μM
0	62 ± 4	56	57	0
0.5	44 ± 4	38	nd	nd
1	30 ± 1	24	50	14
2	6.1 ± 0.5	0	47	15
3	4.7 ± 1.2	0	44	17
5	7.2 ± 0.5	1	42	18
10	4.7 ± 2.0	0	nd	nd
SOR + 5 equivalents ferrocyanide + 50 μM H2O2	54 ± 1	48	nd	nd
No SOR, ferrocyanide 200 μM	34 ± 2	28	/	0

The SOR solution (100 μM) contained various molar equivalents of ferrocyanide compared with the SOR iron active site. Each value represents the mean of at least three independent experiments. nd, not determined; /, not relevant.

*Dosage of H₂O₂ with the leuco crystal violet method.

^†Corrected from the radiolytic yield for H₂O₂ = 6.1 μM.

^‡Determined from the spectra of Fig. 8 by using ε_{644 nm} = 1,900 M⁻¹·cm⁻¹ for the oxidized SOR active site and ε_420nm = 1,010 M⁻¹·cm⁻¹ for the ferricyanide.