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Light (416 nm)-minus-dark FTIR difference spectra of CO-bound sensor domain HemAT proteins at ambient temperatures. Labeling is as follows: trace A, WT; trace B, R91A; trace C, T95A; trace D, Y70F; trace E, L92A; trace F, Y133F. Inset shows the 2,040- to 2,160-cm−1 region ×10.
