FIG. 2.

Collagen binding of the collagenolytic protease from strain MO-1 and bovine serum albumin. The binding assays were carried out according to the method described in Materials and Methods. The collagenolytic protease (2.5 μg) was incubated without any insoluble protein (lane 1) or with collagen (5 mg; lane 2), keratin (5 mg; lane 3), or elastin (5 mg; lane 4) at 0°C for 180 min. Bovine serum albumin (2.5 μg) was incubated in variations corresponding to those of the collagenolytic protease (lanes 1′ to 4′). Numbers at the bottom of the gel are the densitometric ratios of each band compared with that of the control lane.