Nondenaturing PAGE analysis for enzyme reconstituted from mutant subunits. A. Lanes: 1, standard molecular mass markers; 2, CLL plus CLS; 3, CLL plus CLSG56D; 4, CLL plus CLSG57D; 5, CLL plus CLSR58L; 6, CLL plus CLSE97A; 7, CLL plus CLSE104A; 8, CLL plus CLSE188Q; 9, CLL plus CLSN190K; 10, CLL plus D205V. B. Lanes: 1, standard molecular mass markers; 2, CLL plus CLS; 3, CLLS176A plus CLS; 4, CLLG177F plus CLS; 5, CLLG178F plus CLS; 6, CLLE231L plus CLS. Approximately 10 μg of the reconstituted proteins was loaded onto the gel.