FIG. 1.

Phenotype of the CFU obtained from viable plating of P. aeruginosa wild-type cells and the ΔPA4352 mutant on LB agar plates after 9 hours of anaerobic stationary phase. Cultures of P. aeruginosa were incubated anaerobically in LB supplemented with 50 mM KNO₃. After 9 hours of incubation in the stationary phase, aliquots from (A) wild-type P. aeruginosa containing the empty mini-CTX2 vector (NB058), (B) the ΔPA4352 mutant containing the empty mini-CTX2 vector (NB059), and (C) the complemented ΔPA4352 mutant containing pNB011 with the PA4352 gene (NB060) were taken, and 50 μl of a 10⁻⁵ dilution was plated on LB agar plates. The plates were incubated aerobically for 20 h at 37°C.