FIG. 5.

(A) Induction of the P_PA4352-lacZ fusion during anaerobiosis in P. aeruginosa wild type (black bars), the anr mutant (white bars), and the mutated promoter P_PA4352ΔANR-lacZ in wild-type cells (gray bars). The strains were grown at 37°C in LB to an OD₅₇₈ of 0.7 (time zero) and transferred to hermetically sealed bottles for a 4-hour incubation (time 4). β-Galactosidase activities were determined at the indicated time points. (B) Expression patterns of P_PA4352-lacZ in P. aeruginosa wild-type cells (black bars) and the anr mutant (white bars) and the P_PA4352ΔANR-lacZ fusion containing a mutated Anr recognition site in wild-type cells (gray bars). The cells were grown aerobically in LB at 37°C. β-Galactosidase activities were determined in exponential and late stationary phases (see Materials and Methods). All experiments were repeated three times, and standard deviations are indicated.