FIG. 5.

Identification of GL-2 as the substrate for Rv3782. For testing of GL-2 as a putative acceptor for galactose transfer, the reaction mixtures contained 40,000 dpm of dried GL-2; preincubated UDP-Galp (20 nmol) and mutase (20 μg) in 10 μl of buffer A; membranes from control or overproducing strains (0.5 mg), respectively; and buffer A in a final volume of 80 μl. Incubation was carried out for 2 h at 37°C. Extraction of glycolipids was performed as described in Materials and Methods, and the products were analyzed by TLC in CHCl₃-CH₃OH-NH₄OH-1 M ammonium acetate-H₂O (180:140:9:9:23).