TABLE 1.
Oligonucleotides used for preparation of DNA probes, primer extensions, and site-specific mutagenesis
| Oligonucleotide | Sequence |
|---|---|
| traA_fw | CGTCTGAATATGCTTCGCC |
| traA_rev | AGAACCGCTGCACCAATAC |
| degP_fw | CTATGTCGTCACCAACAACC |
| degP_rev | CGTCAACTTTCATCGCTTTC |
| cpxA_fw | TTCAACAATCGCCAGCCC |
| cpxA_rev | AAACTTACGCCAGCACCC |
| motA_fw | TGAACGACCCCCATTACAG |
| motA_rev | GCAAAGCGATTAAAGGCAC |
| groEL_fw | CCATCTCCGCTAACTCCGAC |
| groEL_rev | GCAACCACGCCTTCTTCTAC |
| dnaK_fw | GACGAACCCGCAAAACAC |
| dnaK_rev | TGACCACCAACGAGGATAAC |
| hslU_fw | AACAGTGTGTAAACGACGAG |
| hslU_rev | GGAAGAGATGACCAGCCAG |
| groESL_fw_ext | GACCCAGAATCGCAAACAC |
| groESL_rev_ext | TCTTTACGCTTGACGATCAC |
| Cy5groESL_rev_ext | Cy5-TCTTTACGCTTGACGATCAC |
| rpoH_fw | CCCAGTTATCATCTTCAATGCC |
| rpoH_rev | GCAGCTAAAACGCTGATCC |
| rpoH_fw_ext | CGCCTGAATAATAAAAGCGTG |
| rpoH_rev_ext | CAACTGGGGCTAAAGCTAAAC |
| Cy5rpoH_rev_ext | Cy5-CAACTGGGGCTAAAGCTAAAC |
| Ucas | CAAGAATTGCCGGCGGAT |
| Dcas | GGTATTTCACACCGCATAGC |
| fw_traAhom | TGAAGAAATATTCAGGGAGGTGATCGAAGAGAACGAGTCAACAAAGAATTGCCGGCGGAT |
| rev_traAhom | ACCGCTTGCCATCAGGTCCTGGCCCTGTGCTGCCATTGCCAGTTGGTATTTC ACACCGCATAGC |
| traAmut_au_fw | GGCGGGATAAGCAGAACAG |
| traAmut_au_rev | AATAAATACAGAGATGATGGCAAAAC |
| traAmut_in_fw | TAAACAGAGGAATCATTAGTAGACTGa |
| traAmut_in_rev | CAGTCTACTAATGATTCCTCTGTTTAa |
a
Underlined letters indicate the two stop codons which were introduced into the traA gene for construction of R1-16/A0.