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. 2006 Sep;188(18):6449–6459. doi: 10.1128/JB.00453-06

FIG. 1.

FIG. 1.

(A) The glmS-pstS intergenic region of APEC strain χ7122. Black arrows indicate the four ORFs of the stg gene cluster. Numbers indicate the predicted number of amino acids. White arrows indicate corresponding genes present in E. coli K-12. Gray arrows indicate positions of primers used for cloning the stg encoding region (glmS-F and pstS-R) and for screening the presence of stg sequences among isolates (stgC-F and stgC-R). The small white rectangle corresponds to the region amplified for screening the presence of stg sequences among isolates. The dashed lines indicate the 5′ and 3′ products amplified to assess the presence of a full-length stg gene cluster among isolates. (B) Phylogram based on Clustal analysis of proteins sharing highest identities/similarities with the predicted stgA gene product. The APEC StgA protein is highlighted within a box. Fimbrial proteins from gene clusters inserted in the yhjW-yhjX region, which includes LpfA of serovar Typhimurium, cluster together and belong to the LP fimbrial group. Fimbrial proteins from gene clusters inserted in the glmS-pstS region, including StgA from E. coli and serovar Typhi, comprise a distinct cluster and belong to the Stg fimbrial group. Entries from top to bottom correspond to GenBank accession numbers P43660, C86029, AAO22843, AAO52822, P22595, AAL18161, AAN45247, CAD03135, AAG58930, and CAE14452. The scale indicates percent difference in similarity. E. tarda, Edwardsiella tarda; P. luminescens, Photorhabus luminescens; S. flexneri, Shigella flexneri; S. marcescens, Serratia marcescens.