TABLE 1.
L. monocytogenes strains and plasmids used in this study
| Listeria plasmid or strain | Genotype, description, or sequence | Resistance marker(s)a | Source or reference |
|---|---|---|---|
| Plasmids | |||
| PCR | Gram-negative cloning vector | Ap, Km | Invitrogen |
| pCR -lmo2537 | pCR with lmo2537 | Ap, Km | This study |
| pLIV1 | Gram-negative/gram-positive shuttle vector; thermosensitive | Cm, Em | 15 |
| pLIV-lmo2537 | pLIV1 with lmo2537 | Cm, Em | This study |
| pG1ts | Gram-negative/gram-positive shuttle vector; thermosensitive | Em, Km | P. Trieu-Cuot |
| pG1ts -Δlmo2537 | pG1ts with lmo2537 flanked regions | Em, Km | This study |
| pATgfp7 | pAT28 carrying the gfp gene under psvpA control | Spc | 48 |
| pATgfp/plmo2537 | pAT28 carrying the gfp gene under plmo2537 control | Spc | This study |
| Strains | |||
| E. coli TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen | |
| L. monocytogenes | |||
| EGD-e | Virulent wild-type clinical isolate, serovar 1/2a | 26 | |
| EGDpGΔlmo2537-lmo2537 | EGD-e carrying a chromosomally integrated copy of pG1tsA-Km-B | Em, Cm, Km | This study |
| EGD lmo2537/pLiv-lmo2537 | EGD-e carrying a chromosomal deletion of lmo2537 and plasmid pLIV-lmo2537 | Em, Cm, Km | This study |
a
Cm, chloramphenicol; Em, erythromycin; Ap, ampicillin; Spc, spectinomycin; Km, kanamycin.