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. 2006 Oct;188(19):6728–6738. doi: 10.1128/JB.00586-06

FIG. 4.

FIG. 4.

Complementation shows that hns::kan is not a null allele. Strains carrying the chromosomal bgl-lacZ reporter specific for repression via the downstream site by H-NS and StpA were transformed with a control plasmid (pKETW15) (A) or with plasmids encoding wild-type hns (pKETW13) (B), hns::amp (pKETW14) (C), hns::kan (pKETW8) (D), and hnsKN (the 5′ end of hns::kan; pKETW7) (E) under the control of the tac promoter. All plasmids code for C-terminal His6-tagged H-NS variants. In addition, plasmid pKETW10, encoding an HA-tagged version of H-NSKN, (F) was used. The plasmids also carry the lacIq gene, which is present upstream of the tac promoter. Strains that carry the lacUV5 promoter-bglG(orf)-lacZ reporter include S1195 (wild type) and stpA and hns derivatives (Table 1).