TABLE 1.
Cell length measurements of transformants of M. smegmatis 628-53 containing the complementing alleles generated in this study
| Vector, complementing allele, or WhmD mutation | Cell length (μm)a | % of cells below cutoffb |
|---|---|---|
| Vector (pBP10 zeo) | 23.28 ± 2.2 | 0 |
| M. smegmatis WhmD | 5.16 ± 0.31 | 100 |
| S. coelicolor WhiB | 7.33 ± 0.48 | 65 |
| M. tuberculosis WhiB2 | 6.95 ± 0.5 | 80 |
| M. smegmatis WhmD mutations | ||
| ΔN42 | 4.55 ± 0.39 | 90 |
| ΔN57 | 5.35 ± 0.46 | 90 |
| ΔN62 | 4.39 ± 0.53 | 90 |
| ΔN67 | 7.12 ± 0.44 | 65 |
| ΔC12 | 6.14 ± 0.33 | 76.6 |
| ΔC23 | 10.51 ± 0.6 | 20 |
| ΔC30 | 22.6 ± 1.59 | 0 |
| C67A | 6.43 ± 0.4 | 63 |
| C90A | ND | |
| C93A | 20.1 ± 1.73 | 0 |
| C99A | 19.44 ± 2.49 | 0 |
| D71A | 24.29 ± 2.29 | 3.33 |
| Y102G | 6.95 ± 0.63 | 66.6 |
| Y102P | 5.66 ± 0.38 | 83.3 |
| G111P | 25.7 ± 1.65 | 0 |
| L116P | 13.2 ± 1.18 | 16.6 |
| R122G | 6.12 ± 0.54 | 83.3 |
| R122P | 5.23 ± 0.22 | 93.3 |
a
Values represent means ± standard errors of the means (n = 30). ND, not determined.
b
Percentage of cells below the cell length cutoff of ≤7.5 μm, which defines a filament.