TABLE 1.
Escherichia coli strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Source or reference | ||
|---|---|---|---|---|
| Strains | ||||
| MG1655 | rph-1 | 4 | ||
| DH5α | Δ(lab)U169 φ80 Δ(lacZ)M15 hsdR17 endA1 gyrA96 recA1 supE44 thi-1 | 28 | ||
| JM3733 | WJW45 rna-30 ΔcsdA | 10 | ||
| JM3734 | WJW45 ΔsrmB | 11 | ||
| GN5002 | MG1655 ΔcgtAE::kan + pGK14 | 35 | ||
| GN5003 | MG1655 ΔcgtAE::kan + pGK15 | 35 | ||
| JM1138 | BL21+pJM1138 | 80 | ||
| JM3903 | MG1655 ΔcgtAE::kan + pMJ31 | This work | ||
| JM3907 | MG1655 ΔcgtAE::kan + pMJ32 | This work | ||
| JM4711 | JM3903 + pJW3146 | This work | ||
| JM4714 | JM3907 + pJW3146 | This work | ||
| Plasmids | ||||
| pCR2.1TOPO | ori-PUC kan bla | Invitrogen | ||
| pGD103-bla | ori-pSC101 bla | 16 | ||
| pGK14 | ori-pSC101 cat PBADcgtAE+ | 35 | ||
| pGK15 | ori-pSC101 cat PBADcgtAE(G80E D85N) | 35 | ||
| pJW3146 | PT5-lachis-rrmJ | http://ecoli.aist-nara.ac.jp | ||
| pJM1138 | PT7his-cgtAE | 80 | ||
| pMJ25 | ori-PUC kan bla cgtAE+ | This work | ||
| pJM3738 | ori-PUC kan bla cgtAE(G80E D85N) | This work | ||
| pMJ27 | ori-PUC kan bla PCgtAE | This work | ||
| pMJ33 | ori-pUC kan bla PCgtAEcgtAE+ | This work | ||
| pMJ29 | ori-pUC kan bla PCgtAEcgtAE(G80E D85N) | This work | ||
| pMJ31 | ori-pSC101 bla PCgtAEcgtAE+ | This work | ||
| pMJ32 | ori-pSC101 bla PCgtAEcgtAE(G80E D85N) | This work | ||