TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH10B | recA1 endA1 | GIBCO/BRL |
| ER2566 | lon ompT lacZ::T7 | New England Biolabs |
| S17-1 | recA1 endA1 thi hsdR RP4-2 Tc::Mu::Tn7 Tpr Smr | 51 |
| S. meliloti strains | ||
| RU11/001 | Smr; spontaneous streptomycin-resistant wild-type strain | 42 |
| RU11/555 | Smr; Δrem | This work |
| RU11/557 | Smr; rem D47N | This work |
| RU11/814 | Smr; ΔvisN ΔvisR | This work |
| RU13/006 | Smr; rem D43N | This work |
| RU13/007 | Smr; rem D45N | This work |
| RU13/009 | Smr; rem D45N/D47N | This work |
| RU13/224 | Smr; rem D43N/D45N/D47N | This work |
| Plasmids | ||
| pBBR1MCS-2a | Kmr | 26 |
| pK18mobsacB | Kmr; lacZ mob sacB | 45 |
| pTYB2 | Apr | New England Biolabs |
| pPHU234b | Tcr; promoterless lacZ | 23 |
| pUCBM20 | Apr; lacZ′ | Boehringer |
| pRU2250 | Tcr; promoter of icpA in pPHU235 | 52 |
| pRU2265 | Tcr; promoter of fliF in pPHU234 | 37 |
| pRU2266 | Tcr; promoter of visN in pPHU234 | 37 |
| pRU2269 | Tcr; promoter of motA in pPHU235 | 52 |
| pRU2271 | Tcr; promoter of orf20 in pPHU236 | 37 |
| pRU2273 | Tcr; promoter of flgB in pPHU235 | 37 |
| pRU2274 | Tcr; promoter of flaA in pPHU236 | 52 |
| pRU2278 | Tcr; promoter of orf38 in pPHU236 | 52 |
| pRU2361 | Tcr; promoter of rem in pPHU236 | This work |
| pRU2365 | Apr; 669-bp NdeI-SapI PCR fragment containing rem cloned into pTYB2 | This work |
| pRU2803 | Gmr; 1,490-bp EcoRI-XbaI PCR fragment containing visNR cloned into pBBR1MCS-5 | This work |
| pRU2818 | Kmr; 672-bp BamHI-HindIII PCR fragment containing rem cloned into pBBR1MCS-2 | This work |
| pRU2916 | Apr; 216-bp EcoRI-HindIII PCR fragment containing PflgB cloned into pUCBM20 | This work |
| pRU2917 | Apr; 309-bp EcoRI-HindIII PCR fragment containing PfliF cloned into pUCBM20 | This work |
| pRU2918 | Apr; 252-bp EcoRI-HindIII PCR fragment containing Porf38 cloned into pUCBM20 | This work |
| pRU2919 | Apr; 338-bp EcoRI-HindIII PCR fragment containing Prem cloned into pUCBM20 | This work |
a
pBBR1MC5-5 is a pBBR1MCS-2 derivative with a different multiple-cloning site and resistance marker (26).
b
pPHU235 and pPHU236 are pPHU234 derivatives with different translational phasing of lacZ (23).