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Thiamine pyrophosphate biosynthetic pathway in S. enterica. (A) Simplification of the biosynthetic pathway for thiamine production. Emphasis is placed on the two reactions relevant to this work. The reactions are catalyzed by the gene product indicated. The PurD reaction has been used in the coupled assay to monitor PRA formation in vitro (36). (B) Reaction catalyzed by the TrpDE enzyme complex that results in an intermediate in the biosynthesis of tryptophan.
