TABLE 3.

Neither the trpE3613 allele nor a yjgF null mutation affects the catalytic activities of the AS-PRT complex

Strain	Relevant genotype	Activitya
		AS (NH4)b		AS (Gln)c		PRTd
		No Trp	Trp	No Trp	Trp	No Trp	Trp
DM7436	purF gnd yjgF	0.29 ± 0.04	0.03 ± 0.01	0.32 ± 0.03	0.10 ± 0.01	0.30 ± 0.06	0.20 ± 0.05
DM7435	purF gnd yjgF trpE3613	0.27 ± 0.02	0.25 ± 0.01	0.31 ± 0.04	0.28 ± 0.01	0.33 ± 0.05	0.20 ± 0.03
DM8126	purF gnd trpE3613	0.28 ± 0.04	0.24 ± 0.00	0.38 ± 0.09	0.34 ± 0.05	0.20 ± 0.03	0.14 ± 0.01
DM7435	purF gnd yjgF trpE3613	0.28 ± 0.09	0.24 ± 0.07	0.33 ± 0.10	0.31 ± 0.10	0.27 ± 0.07	0.12 ± 0.06

^aStrains were grown in minimal glucose medium supplemented with adenine, thiamine, and acid-hydrolyzed casein, as described in the Materials and Methods. Assays were performed with cell extracts in the presence and absence of tryptophan.

^bAS (NH₄), NH₄-dependent anthranilate synthase activity. One unit of activity is defined as the appearance of 1 nmol of anthranilate in 1 min per mg of protein.

^cAS (Gln), glutamine-dependent anthranilate synthase activity. One unit of activity is defined as the appearance of 1 nmol of anthranilate in 1 min per mg of protein.

^dPRT, phosphoribosyl transferase. One unit of activity is defined as the disappearance of 1 nmol of anthranilate in 1 min per mg of protein.