FIG. 7.

Expression of glnA2 is stimulated by chloride. H. halophilus was cultivated in glucose mineral salt medium in the presence of NaCl, sodium gluconate (Na-gluc), or NaNO₃ at a final concentration of 1.0 M. Total RNA was isolated and transcribed into cDNA, and relative transcription levels were measured using real-time PCR analysis. In the diagram, relative quantitation of transcript levels is given. All values were compared to the value for NaCl, which was defined as 1. The experiment was repeated in three independent parallels to ensure statistical relevance.