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. 2006 Oct;188(19):6877–6888. doi: 10.1128/JB.00536-06

FIG. 5.

FIG. 5.

Effect of ThmA on the conversion of AtlA from a 107-kDa to a 79-kDa peptide. (A) Protein profiles in Coomassie-stained SDS-PAGE gel of UA159 (wild type; lane 1) and its derivatives, SAB66 (ΔpepT, polar mutant; lane 2), SAB67 (ΔpepT, nonpolar mutant; lane 3), and SAB71 (ΔthmA mutant; lane 4). (B) Effect of ThmA on the processing of exogenous 630D1. Two strains, 630NP (lane 1 and 2) and SAB78 (lanes 3 and 4), were grown in BHI broth; samples either were left untreated (lanes 1 and 3), or purified 630D1 was added to a final concentration of 20 ng/ml culture for 1 h (lanes 2 and 4). Following SDS-PAGE, proteins were stained with Coomassie blue (top) or transferred onto a nitrocellulose membrane and subjected to Western blotting using an anti-630D1 polyclonal antiserum (dilution 1:350) (bottom). Lane M, size marker. WT, wild type.