TABLE 2.
Summary of genes showing different expression levels between strains W1485 and HBA-1 grown in glucose-limited chemostat culture, as revealed by DNA array analysisa
| Gene | Gene product | Avg spot intensityb for:
|
SD for:
|
Ratio (HBA-1/W1485)c | ||
|---|---|---|---|---|---|---|
| W1485 | HBA-1 | W1485 | HBA-1 | |||
| aceE | Pyruvate dehydrogenase E1 component | 1.18E−04 | 1.48E−04 | 2.09E−05 | 3.27E−05 | 1.26 |
| aceF | E2 of pyruvate dehydrogenase | 1.52E−04 | 1.92E−04 | 2.79E−05 | 3.52E−05 | 1.26d |
| gltA | Citrate synthase | 4.05E−04 | 1.80E−04 | 7.80E−05 | 6.03E−05 | 0.45d |
| icdA | Isocitrate dehydrogenase | 2.95E−04 | 1.80E−04 | 4.53E−05 | 2.27E−05 | 0.61d |
| sucA | 2-Oxoglutalate dehydrogenase E1 component | 1.34E−04 | 7.63E−05 | 3.45E−05 | 2.25E−05 | 0.57d |
| sucB | Dihydrolipoamide succinyltransferase component (E2) | 1.29E−04 | 6.19E−05 | 2.94E−05 | 1.52E−05 | 0.48d |
| sucD | Succinyl-CoA synthetase α chain | 4.86E−04 | 1.72E−04 | 1.87E−04 | 8.94E−05 | 0.35d |
| mdh | Malate dehydrogenase | 3.18E−04 | 1.34E−04 | 1.14E−04 | 3.66E−05 | 0.42d |
| aceA | Isocitrate lyase | 2.37E−04 | 1.52E−04 | 5.90E−05 | 3.01E−04 | 0.64d |
| aceB | Malate synthase A | 3.82E−04 | 2.22E−04 | 7.72E−05 | 9.11E−05 | 0.58d |
| ndh | NADH dehydrogenase II | 8.25E−05 | 1.09E−04 | 1.18E−05 | 1.94E−05 | 1.32d |
| nuoA | NADH dehydrogenase I chain A | 5.78E−05 | 4.96E−05 | 1.27E−05 | 8.07E−06 | 0.86 |
| cydA | Cytochrome d oxidase subunit I | 3.00E−04 | 4.02E−04 | 1.61E−04 | 2.43E−04 | 1.34d |
| cyoA | Cytochrome o oxidase subunit II | 3.24E−04 | 4.19E−04 | 1.51E−04 | 2.49E−04 | 1.30 |
| flhC | Flagellar transcriptional activator | 1.99E−04 | 8.96E−05 | 4.14E−07 | 8.26E−06 | 0.45d |
| flhD | Flagellar transcriptional activator | 2.57E−04 | 1.12E−04 | 2.68E−07 | 2.28E−05 | 0.44d |
| fliC | Flagellin | 8.68E−04 | 3.64E−04 | 4.55E−05 | 1.00E−04 | 0.42d |
| fliD | Flagellar hook-associated protein 2 | 1.43E−04 | 8.07E−05 | 1.68E−05 | 2.70E−05 | 0.56d |
| flgB | Putative flagellar basal-body rod protein FlgB | 3.19E−04 | 1.85E−04 | 1.85E−05 | 6.18E−05 | 0.58d |
| flgC | Putative flagellar basal-body rod protein FlgC | 1.52E−04 | 8.88E−05 | 6.24E−06 | 2.77E−05 | 0.58d |
| flgL | Flagellar hook-associated protein 3 | 2.18E−04 | 1.20E−04 | 9.01E−06 | 1.79E−05 | 0.55d |
| ompF | Outer membrane protein F precursor | 2.58E−03 | 1.21E−03 | 5.83E−04 | 3.00E−04 | 0.47d |
| nmpC | Outer membrane porin protein NmpC precursor | 1.60E−04 | 1.10E−04 | 5.19E−05 | 1.84E−05 | 0.69d |
| hns | Histone-like protein H-NS | 1.20E−04 | 6.06E−04 | 3.18E−04 | 3.57E−04 | 0.50d |
a
The results are from eight independent DNA array analyses.
b
Expressed as the percentage of the total intensity of all the spots on the DNA array.
c
Expression ratio of HBA-1 to W1485.
d
Significant t test differences (P < 0.05).