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Comparison of real-time PCR- and GeneChip-based mRNA half-life determinations
| Assay | mRNA half-life (min)
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Cold shock
|
Heat shock
|
Stringent response
|
SOS response
|
|||||
| srtA | cspB | clpC | ctsR | sarR | norA | recA | uvrB | |
| GeneChip microarray | 15-30 | ≥30 | ≥30 | ≥30 | 15-30 | ≥30 | ≤2.5 | ≤2.5 |
| Real-time PCR | 30 | 30 | 15 | 15-30 | 15 | 15-30 | 2.5 | 2.5 |
