FIG. 3.

In vitro transcription analysis of phyC. Transcription was carried out for 20 min at 37°C with purified RNAP in various amounts and a 10 nM concentration of a 511-bp phyC template of the wild-type (A) or MUT13 (B) promoter. PhoP (1.25 μM) was phosphorylated by 0.3 μM PhoR in binding buffer in the presence of 5 mM ATP for 20 min as described previously and was then added to the transcription reaction. The final concentrations (nM) of RNAP and PhoP-P are given at the top. M, molecular standard.