FIG. 4.

Deletion analysis of the FZB45 phyC promoter. Top: fusion product consisting of the phyC promoter linked at +208 with the lacZ gene. Position of the PhoP boxes, of the −35 and −10 promoter sequences, and of the start point of transcription (+1) are indicated. The filled boxes represent the various lengths of the phyC promoter fragments used in this assay. The 5′ and 3′ ends of each fragment were labeled relative to the transcription start site, +1. The strains carrying the various truncated phyC promoters were grown in LPM, and the promoter activity was determined every hour. The highest activity of the reporter was obtained after 6 h and was used for calculating the relative promoter activity. The reporter activity of the full-length promoter corresponds to 100%; the activities of the other promoters are calculated as the average percentages of expression relative to that of the full-length promoter. The average mean deviation (±) was calculated from three independent experiments.