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. Author manuscript; available in PMC: 2006 Oct 11.
Published in final edited form as: J Periodontol. 2004 Feb;75(2):283–291. doi: 10.1902/jop.2004.75.2.283

Figure 1.

Figure 1

Inerleukin-4 (IL-4) inhibits IL-1 induction of MMP-3 mRNA in human gingival fibroblasts (HGF). Total RNA was isolated from untreated HGF and cells treated for the indicated times with IL-1β alone (100 ng/ml) or in combination with 10 ng/ml IL-4. A. Northern blots were hybridized to cDNA probes corresponding to MMP-3 and GAPDH. B. Blots were quantitated by scanning densitometry and normalized to levels of GAPDH. Shown are data from three independent experiments, each of which utilized RNA isolated from three pooled HGF cultures established from three different donors. (* p< 0.05 vs. IL-1 alone).