Skip to main content
. Author manuscript; available in PMC: 2006 Oct 11.
Published in final edited form as: J Periodontol. 2004 Feb;75(2):283–291. doi: 10.1902/jop.2004.75.2.283

Figure 6.

Figure 6

DNA binding of transcription factors activator protein-1 (AP-1) and nuclear factor-κB (NF-κB) is not affected by IL-4. Nuclear extracts were isolated from HGF cultures treated for one hour with 100 ng/ml IL-1β (lane 3), 10 ng/ml IL-4 (lane 4) or both IL-1 and IL-4 (lane 5), as well as from control cultures (lane 2). Lane 1, probe alone, no nuclear extract. Binding of 5 μg nuclear extract to 32P-labeled oligo(dT) probes corresponding to consensus AP-1 and NF-κB binding sites were determined by electrophoretic mobility shift assay.